ECM Kits/Skin dECM

Skin dECM

Name: Skin dECM
Brand: xylyx-dev
SKU: MTSSK101
Price: 149.0 USD
Availability: InStock

ECM Kits/Skin dECM

Skin Decellularized Extracellular Matrix (dECM) Kit

Choose your format: TissueSpec® Skin dECM Hydrogel Kit (1 mL) $ 149.00 TissueSpec® Skin dECM Coating Kit (10 mL) $ 59.00

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TissueSpec® Skin dECM Hydrogel Kit (1 mL)

TissueSpec® Skin dECM Coating Kit (10 mL)

Skin Decellularized ECM Hydrogel Kit contents

2 x 0.3 mL TissueSpec® Skin dECM

1 x 1 mL Component A

1 x 1 mL Component B

Download supporting Skin ECM hydrogel protocols

Instructions Data Sheet

Skin ECM hydrogel preparation workflow

Download supporting Skin ECM hydrogel protocols

Dissociation of TissueSpec® dECM Hydrogel for Cell Isolation and Analysis

Preparation of TissueSpec® dECM Hydrogel for Immunostaining

Preparation of Hybrid TissueSpec® dECM and Collagen I Hydrogel

Skin dECM Material properties

Origin: Skin (porcine)

Composition: Characterized by mass spectrometry

Stiffness: 50 – 200 Pa

Usage of extracellular matrix in skin

Use: 3D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 1 mL

Number of wells: 18 wells at 50 µL/well

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: Human primary dermal fibroblasts, melanoma cells (A-375)

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Disease modeling, drug testing, organ-on-chip, organoids, spheroids, stem cells

Quality of the skin ECM hydrogel

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 6 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: 30 minutes (at 37ºC)

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Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

Skin Decellularized ECM Coating Kit contents

1 x 1 mL TissueSpec® Skin dECM (1 mg/mL)

1 x 1 mL 10x Buffer

Download supporting Skin ECM coating protocols

Instructions Data Sheet

Skin ECM coating workflow

Skin dECM Material properties

Origin: Skin (porcine)

Composition: Characterized by mass spectrometry (link to datasheet)

Stiffness: ##

Usage of extracellular matrix in skin

Use: 2D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 10 mL

Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: Human primary dermal fibroblasts, melanoma cells (A-375)

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Drug testing, disease modeling, high throughput screening

Quality of the skin ECM coating

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 0.1 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: N/A

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

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TissueSpec® Skin dECM Kits

TissueSpec® Skin dECM Kits bring the skin matrix environment derived from skin to your cell culture.

TissueSpec® dECM Hydrogel Kit

Each TissueSpec® dECM Hydrogel Kit is lot-to-lot consistent and is sufficient to prepare 1 mL of hydrogel at a concentration of 6 mg/mL. Research use only.

Kit contents include:

2 x 0.3 mL TissueSpec® dECM

1 x 1 mL Component A

1 x 1 mL Component B

TissueSpec® Skin dECM Hydrogel Kit: Morphology of melanoma cells (A375).

Melanoma cells, A375 (5x104) seeded on thin TissueSpec® Skin dECM Hydrogels show their regular morphology not observed in cells seeded on Matrigel.

TissueSpec® Skin dECM Hydrogel Kit: Viability of melanoma cells (A375).

Melanoma cells, A375 (5x103) seeded on 96-well plates were treated with 1𝜇M and 10𝜇M of Vemurafenib for 24 hours. Plastic (no ECM) was used as control condition. Viability was measured using XTT Cell Viability Assay.

TissueSpec® Skin dECM Hydrogel Kit: Migration assay of melanoma cells (A375).

Melanoma cells, A375 (1x105) were seeded on transwells (pore: 8uM) for 18 hours. Bottom wells were seeded with thin TissueSpec® Skin dECM Hydrogels or Matrigel. Plastic (no ECM) was used as the substrate control condition.

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Skin dECM

Skin Decellularized Extracellular Matrix (dECM) Kit

Skin Decellularized ECM Hydrogel Kit contents

Download supporting Skin ECM hydrogel protocols

Skin ECM hydrogel preparation workflow

Download supporting Skin ECM hydrogel protocols

Skin dECM Material properties

Usage of extracellular matrix in skin

Quality of the skin ECM hydrogel

Ordering

Skin Decellularized ECM Coating Kit contents

Download supporting Skin ECM coating protocols

Skin ECM coating workflow

Skin dECM Material properties

Usage of extracellular matrix in skin

Quality of the skin ECM coating

Ordering

TissueSpec® Skin dECM Kits

TissueSpec® dECM Hydrogel Kit

TissueSpec® Skin dECM Hydrogel Kit: Morphology of melanoma cells (A375).

TissueSpec® Skin dECM Hydrogel Kit: Viability of melanoma cells (A375).

TissueSpec® Skin dECM Hydrogel Kit: Migration assay of melanoma cells (A375).

TissueSpec® Skin dECM Kits

TissueSpec® Skin dECM Coating Kit: Morphology of normal dermal fibroblasts.

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