ECM Kits/Skin dECM

Skin dECM

Name: Skin dECM
Brand: xylyx-dev
SKU: MTSSK101
Price: 149.0 USD
Availability: InStock

ECM Kits/Skin dECM

Skin dECM

Choose your format: TissueSpec® Skin dECM Hydrogel Kit (1 mL) $ 149.00 TissueSpec® Skin dECM Coating Kit (10 mL) $ 59.00

Product variants

$ 149.00

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TissueSpec® Skin dECM Hydrogel Kit (1 mL)

TissueSpec® Skin dECM Coating Kit (10 mL)

Kit contents

2 x 0.3 mL TissueSpec® Skin dECM

1 x 1 mL Component A

1 x 1 mL Component B

Downloads

Instructions Data Sheet

Workflow overview

Download supporting protocols

Dissociation of TissueSpec® dECM Hydrogel for Cell Isolation and Analysis

Preparation of TissueSpec® dECM Hydrogel for Immunostaining

Preparation of Hybrid TissueSpec® dECM and Collagen I Hydrogel

Material properties

Origin: Skin (porcine)

Composition: Characterized by mass spectrometry

Stiffness: ##

Usage

Use: 3D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 1 mL

Number of wells: 18 wells at 50 µL/well

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: Human primary dermal fibroblasts, melanoma cells (A-375)

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Disease modeling, drug testing, organ-on-chip, organoids, spheroids, stem cells

Quality

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 6 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: 30 minutes (at 37ºC)

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

Kit contents

1 x 1 mL TissueSpec® Skin dECM

1 x 1 mL 10x Buffer

Downloads

Instructions Data Sheet

Workflow overview

Material properties

Origin: Skin (porcine)

Composition: Characterized by mass spectrometry (link to datasheet)

Stiffness: ##

Usage

Use: 2D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 10 mL

Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: Human primary dermal fibroblasts, melanoma cells (A-375)

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Drug testing, disease modeling, high throughput screening

Quality

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 0.1 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: N/A

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

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TissueSpec® Skin dECM Kits

TissueSpec® Skin dECM Kits bring the skin matrix environment derived from skin to your cell culture.

TissueSpec® Skin dECM Hydrogel Kit: Morphology of melanoma cells (A375).

Melanoma cells, A375 (5x104) seeded on thin TissueSpec® Skin dECM Hydrogels show their regular morphology not observed in cells seeded on Matrigel.

TissueSpec® Skin dECM Hydrogel Kit: Viability of melanoma cells (A375).

Melanoma cells, A375 (5x103) seeded on 96-well plates were treated with 1𝜇M and 10𝜇M of Vemurafenib for 24 hours. Plastic (no ECM) was used as control condition. Viability was measured using XTT Cell Viability Assay.

TissueSpec® Skin dECM Hydrogel Kit: Migration assay of melanoma cells (A375).

Melanoma cells, A375 (1x105) were seeded on transwells (pore: 8uM) for 18 hours. Bottom wells were seeded with thin TissueSpec® Skin dECM Hydrogels or Matrigel. Plastic (no ECM) was used as the substrate control condition.

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Skin dECM

Skin dECM

Kit contents

Downloads

Workflow overview

Download supporting protocols

Material properties

Usage

Quality

Ordering

Kit contents

Downloads

Workflow overview

Material properties

Usage

Quality

Ordering

TissueSpec® Skin dECM Kits

TissueSpec® Skin dECM Hydrogel Kit: Morphology of melanoma cells (A375).

TissueSpec® Skin dECM Hydrogel Kit: Viability of melanoma cells (A375).

TissueSpec® Skin dECM Hydrogel Kit: Migration assay of melanoma cells (A375).

TissueSpec® Skin dECM Kits

TissueSpec® Skin dECM Coating Kit: Morphology of normal dermal fibroblasts.

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