ECM Kits/Lung Fibrotic dECM

Lung Fibrotic dECM

ECM Kits/Lung Fibrotic dECM

Lung Fibrotic dECM

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TissueSpec® Human Lung Fibrotic dECM Coating Kit (10 mL)
Custom TissueSpec® Human Lung Fibrotic dECM Coating Kit (3 donors)

Product Number: MTHDLG201

Size: 10 mL

TissueSpec® Human Lung Fibrotic dECM Coating Kit brings the fibrotic lung matrix environment derived from human lungs to your 2D culture. Each lot is specific to 1 donor and is sufficient to prepare 10 mL of coating at a concentration of 0.1 mg/mL. Research use only.

Kit contents

1 x 1 mL TissueSpec® Human Lung Fibrotic dECM

1 x 1 mL 10x Buffer

Workflow overview

Graphical workflow

Material properties

Origin: Lung (human)

Composition: Characterized by mass spectrometry

Stiffness: ##

Usage

Use: 2D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 10 mL

Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: Primary lung fibroblasts, human primary bronchial epithelial cells, small airway epithelial cells (SAECs), A-549 cells, primary lung adenocarcinoma cells, breast cancer cells (BT-549, T47-D), HUVECs, mesenchymal stem cells

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Drug testing, disease modeling, high throughput screening

Quality

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 0.1 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: N/A

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

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