ECM Kits/Lung dECM

Lung dECM

Name: Lung dECM
Brand: xylyx-dev
SKU: MTSLG101
Price: 149.0 USD
Availability: InStock

ECM Kits/Lung dECM

Lung dECM

Choose your format: TissueSpec® Lung dECM Hydrogel Kit (1 mL) $ 149.00 TissueSpec® Lung dECM Coating Kit (10 mL) $ 59.00

Product variants

$ 149.00

Sale Sold out

TissueSpec® Lung dECM Hydrogel Kit (1 mL)

TissueSpec® Lung dECM Coating Kit (10 mL)

Kit contents

2 x 0.3 mL TissueSpec® Lung ECM

1 x 1mL Component A

1 x 1mL Component B

Downloads

Instructions Data Sheet

Workflow overview

Download supporting protocols

Dissociation of TissueSpec® dECM Hydrogel for Cell Isolation and Analysis

Preparation of TissueSpec® dECM Hydrogel for Immunostaining

Preparation of Hybrid TissueSpec® dECM and Collagen I Hydrogel

Material properties

Origin: Lung (porcine)

Composition: Characterization by mass spectrometry

Stiffness: ## Pa

Usage

Use: 3D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 1 mL

Number of wells: 18 wells at 50 µL/well

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: Primary lung fibroblasts, human primary bronchial epithelial cells, small airway epithelial cells (SAECs), A-549 cells, primary lung adenocarcinoma cells, breast cancer cells (BT-549, T47-D), HUVECs, mesenchymal stem cells

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Disease modeling, drug testing, organ-on-chip, organoids, spheroids, stem cells

Quality

Biocompatibility: >95% viability of human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 6 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: 30 minutes (at 37ºC)

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

Kit contents

1 x 1mL TissueSpec® Lung ECM

1 x 1mL 10x Buffer

Downloads

Instructions Data Sheet

Workflow overview

Material properties

Origin: Lung (porcine)

Composition: Characterized by mass spectrometry

Stiffness: ##

Usage

Use: 2D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 10 mL

Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)

Storage: 2–8ºC

Shelf life: 12 months

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Drug testing, disease modeling, high throughput screening

Quality

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 0.1 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: N/A

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

Scroll to top

TissueSpec® Lung dECM Kits

TissueSpec® Lung dECM Kits bring the lung matrix environment derived from lungs to your cell culture.

TissueSpec® Lung dECM Hydrogel Kit: Quantification of p63+ cells.

Number of p63+ cells were higher on thin gels of of lung hydrogels (6mg/mL) than Matrigel.

TissueSpec® Lung dECM Hydrogel Kit: Primary normal human bronchial epithelial cells.

Primary normal human bronchial epithelial cells cultured on thin layers of TissueSpec® Lung dECM Hydrogel (6mg/mL) or Matrigel for 10 days. Normal lung epithelial markers p63 (red) and ZO-1 (green) were used.

TissueSpec® Lung dECM Hydrogel Kit: Luminal formation.

Air liquid interface (ALI) cultures using primary normal human bronchial epithelial cells in TissueSpec® Lung dECM Hydrogels showed a more complex and organized structure compared to Matrigel.

TissueSpec® Lung dECM Hydrogel Kit: Structural organization of primary normal human bronchial epithelial cells.

Air liquid interface (ALI) cultures using primary normal human bronchial epithelial cells in TissueSpec® Lung dECM Hydrogels showed a more organized, stratified luminal structures with significantly larger average diameter compared to Matrigel.

Cart

Lung dECM

Lung dECM

Kit contents

Downloads

Workflow overview

Download supporting protocols

Material properties

Usage

Quality

Ordering

Kit contents

Downloads

Workflow overview

Material properties

Usage

Quality

Ordering

TissueSpec® Lung dECM Kits

TissueSpec® Lung dECM Hydrogel Kit: Quantification of p63+ cells.

TissueSpec® Lung dECM Hydrogel Kit: Primary normal human bronchial epithelial cells.

TissueSpec® Lung dECM Hydrogel Kit: Luminal formation.

TissueSpec® Lung dECM Hydrogel Kit: Structural organization of primary normal human bronchial epithelial cells.

TissueSpec® Lung dECM Kits

TissueSpec® Lung dECM Coating Kit: Morphology of lung adenocarcinoma cells.

TissueSpec® Lung dECM Coating Kit: Morphology of breast cancer cells.

Get in touch