TissueSpec® Lung dECM Kits bring the lung matrix environment derived from lungs to your cell culture.

Each TissueSpec® dECM Hydrogel Kit is lot-to-lot consistent and is sufficient to prepare 1 mL of hydrogel at a concentration of 6 mg/mL. Research use only.

Kit contents include:

2 x 0.3 mL TissueSpec® dECM

1 x 1 mL Component A

1 x 1 mL  Component B

Patient-derived breast cancer organoids (ATCC No. PDM-92™) were used to model tumor growth in breast-to-lung metastasis over 11 days. Scale bar = 1000 µm.

Patient-derived breast cancer organoids (ATCC No. PDM-92™) were used to model tumor growth in breast-to-lung metastasis over 11 days. Scale bar = 100 µm.

BT549 human breast cancer spheroids were embedded in TissueSpec® Lung dECM Hydrogel to model cell invasion in a secondary site of metastasis and for subsequent transcriptomic analysis. Breast cancer spheroids were imaged at Day 0, 3, and 7. Yellow arrowheads denote tumor cells that have disseminated from the spheroid to initiate invasion into the surrounding lung dECM microenvironment. Scale bar = 100 µm.

BT549 human breast cancer spheroids were embedded in either TissueSpec® Lung dECM Hydrogel or Matrigel for 3 days to assess the influence of two distinct extracellular matrix environments on tumor cell gene expression. After incubation, RNA was extracted from BT549 spheroids for transcriptomic analysis and a total of 3,181 genes were found to be differentially expressed (p ≤ 0.05) between the two conditions. Of these, 453 genes were significantly upregulated and 1,259 genes were significantly downregulated (p ≤ 0.01) in the TissueSpec® Lung dECM Hydrogel compared to Matrigel.

Volcano plot illustrating differential gene expression between BT549 human breast cancer spheroids cultured in TissueSpec® Lung dECM Hydrogel versus Matrigel. Red dots represent significantly upregulated genes (p ≤ 0.01), blue dots indicate significantly downregulated genes (p ≤ 0.01), and purple dots denote genes meeting the overall threshold for differential expression (p ≤ 0.05). Notable genes enriched in TissueSpec® Lung dECM Hydrogel include COL1A1, MXRA5, CD109, and SLC16A2, while CKS2, ZFA1, and KIRREL-IT1 were among the most downregulated.

Primary normal human bronchial epithelial cells cultured on thin layers of TissueSpec® Lung dECM Hydrogel (6mg/mL) or Matrigel for 10 days. Normal lung epithelial markers p63 (red) and ZO-1 (green) were used.

Number of p63+ cells were higher on thin gels of of lung hydrogels (6mg/mL) than Matrigel.

Air liquid interface (ALI) cultures using primary normal human bronchial epithelial cells in TissueSpec® Lung dECM Hydrogels showed a more complex and organized structure compared to Matrigel.

Air liquid interface (ALI) cultures using primary normal human bronchial epithelial cells in TissueSpec® Lung dECM Hydrogels showed a more organized, stratified luminal structures with significantly larger average diameter compared to Matrigel.

TissueSpec® Lung dECM Kits bring the lung matrix environment derived from lungs to your cell culture.

Lung adenocarcinoma cells (5x10^4) were seeded on 12-well plates previously coated using TissueSpec® Lung dECM Coating (0.2mg/mL) for 48 hours.

Lung adenocarcinoma cells (5x10^4) were seeded on 12-well plates previously coated using TissueSpec® Lung dECM Coating (0.2mg/mL) for 48 hours.