ECM Kits/Liver Fibrotic dECM
Liver Fibrotic dECM
ECM Kits/Liver Fibrotic dECM
Liver Fibrotic dECM
Kit contents
1 x 1 mL TissueSpec® Human Liver Fibrotic dECM (1 mg/mL)
1 x 1 mL 10x Buffer
Material properties
Origin: Liver (human)
Composition: Characterized by mass spectrometry
Stiffness: ##
Usage
Use: 2D cell culture
Preparation temperature: 25ºC
Preparation time: 15 min
Hydrogel volume: 10 mL
Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)
Storage: 2–8ºC
Shelf life: 12 months
Validated cell types: primary human hepatocytes, hepatocarcinoma cells (HepG2), primary human hepatic stellate cells, breast cancer cells (BT-549, T47-D), fibroblasts
Validated multi-well formats: 6, 12, 24, 48, 96, 384
Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence
Applications: Drug testing, disease modeling, high throughput screening
Quality
Biocompatibility: >95% viability human primary fibroblasts
Sterility: Negative for bacteria, fungus, mycoplasma
Concentration: 0.1 mg/mL (recommended working concentration)
pH: Neutral
Gelation time: N/A
Ordering
Lead time: 5 business days
Shipping temperature: 4ºC (wet ice, natural insulating material)
TissueSpec® Human Liver Fibrotic dECM Coating Kit (10 mL)
TissueSpec® Human Liver Fibrotic dECM Coating Kit
TissueSpec® Human Liver Fibrotic dECM Kits bring the fibrotic liver matrix environment derived from human livers to your cell culture.
TissueSpec® Human Liver Fibrotic dECM Coating Kit: Fluorescent labeling of fibrotic liver dECM protein fibers
TissueSpec® Human Liver Fibrotic dECM and TissueSpec® Liver dECM Coating were labeled with Alexa Fluor™ 647 NHS Ester to visualize protein distribution in a 12-well plate and imaged at 20x magnification using the Cy5 channel on a Cytation™ 5 imaging system (Agilent BioTek, USA). Scale bar = 150 µm.