ECM Kits/Kidney dECM
Kidney dECM
Kit contents
2 x 0.3 mL TissueSpec® Kidney dECM
1 x 1 mL Component A
1 x 1 mL Component B
Downloads
InstructionsWorkflow overview
Download supporting protocols
Material properties
Origin: Kidney (porcine)
Composition: Characterization by mass spectrometry
Stiffness: ## Pa
Usage
Use: 3D cell culture
Preparation temperature: 25ºC
Preparation time: 15 min
Hydrogel volume: 1 mL
Number of wells: 18 wells at 50 µL/well
Storage: 2–8ºC
Shelf life: 12 months
Validated cell types: kidney stem cells, renal tubule epithelial cells, HUVECs, fibroblasts, mesenchymal stem cells
Validated multi-well formats: 6, 12, 24, 48, 96, 384
Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence
Applications: Disease modeling, drug testing, organ-on-chip, organoids, spheroids, stem cells
Quality
Biocompatibility: >95% viability of human primary fibroblasts
Sterility: Negative for bacteria, fungus, mycoplasma
Concentration: 6 mg/mL (recommended working concentration)
pH: Neutral
Gelation time: 30 minutes (at 37ºC)
Ordering
Lead time: 5 business days
Shipping temperature: 4ºC (wet ice, natural insulating material)
Kit contents
1 x 1mL TissueSpec® Kidney ECM
1 x 1mL 10x Buffer
Workflow overview
Material properties
Origin: Kidney (porcine)
Composition: Characterized by mass spectrometry
Stiffness: ##
Usage
Use: 2D cell culture
Preparation temperature: 25ºC
Preparation time: 15 min
Hydrogel volume: 10 mL
Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)
Storage: 2–8ºC
Shelf life: 12 months
Validated cell types: kidney stem cells, renal tubule epithelial cells, HUVECs, fibroblasts, mesenchymal stem cells
Validated multi-well formats: 6, 12, 24, 48, 96, 384
Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence
Applications: Drug testing, disease modeling, high throughput screening
Quality
Biocompatibility: >95% viability human primary fibroblasts
Sterility: Negative for bacteria, fungus, mycoplasma
Concentration: 0.1 mg/mL (recommended working concentration)
pH: Neutral
Gelation time: N/A
Ordering
Lead time: 5 business days
Shipping temperature: 4ºC (wet ice, natural insulating material)
TissueSpec® Kidney dECM Hydrogel Kit (1 mL)
TissueSpec® Kidney dECM Kits
TissueSpec® Kidney dECM Kits bring the kidney matrix environment derived from kidneys to your cell culture.
TissueSpec® Kidney dECM Hydrogel Kit: Growth of kidney stem cells.
Kidney stem cells were cultured under standard culture conditions, passaged and added to TissueSpec® Kidney dECM Hydrogels at an initial cell density of 2.5x104cells/mL, and cultured for up to 7 days. Morphology (left picture) and structure formation (right picture) in TissueSpec® Kidney dECM Hydrogels were photographed.
TissueSpec® Kidney dECM Coating Kit (10 mL)
TissueSpec® Kidney dECM Kits
TissueSpec® Kidney dECM Kits bring the kidney matrix environment derived from kidneys to your cell culture.
TissueSpec® Kidney dECM Coating Kit: Cobblestone morphology.
Kidney stem cells isolated from renal papilla were cultured under standard culture conditions and added to TissueSpec® Kidney dECM Coating (0.2mg/mL) at an initial cell density of 2.5x104cells/mL and cultured for up to 7 days.