ECM Kits/Intestine dECM

Intestine dECM

ECM Kits/Intestine dECM

Intestine dECM

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TissueSpec® Intestine dECM Hydrogel Kit (1 mL)
TissueSpec® Intestine dECM Coating Kit (10 mL)

Product Number: MTSIN101

Size: 1 mL

TissueSpec® Intestine dECM Hydrogel Kit brings the intestine matrix environment derived from intestines to your 3D culture. Each kit is lot-to-lot consistent and is sufficient to prepare 1 mL of hydrogel at a concentration of 6 mg/mL. Research use only.

Product Number: MTSIN201

Size: 10 mL

TissueSpec® Intestine dECM Coating Kit brings the intestine matrix environment derived from intestines to your 2D culture. Each kit is lot-to-lot consistent and is sufficient to prepare 10 mL of coating at a concentration of 0.1 mg/mL. Research use only.

Kit contents

2 x 0.3 mL TissueSpec® Intestine dECM

1 x 1 mL Component A

1 x 1 mL  Component B

Workflow overview

Graphical workflow

Material properties

Origin: Intestine (porcine)

Composition: Characterization by mass spectrometry

Stiffness: ## Pa

Usage

Use: 3D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 1 mL

Number of wells: 18 wells at 50 µL/well

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: iPSC-derived human intestine organoids, mouse intestinal crypts, CaCo2 cells, fibroblasts, HUVECs, mesenchymal stem cells

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Disease modeling, drug testing, organ-on-chip, organoids, spheroids, stem cells

Quality

Biocompatibility: >95% viability of human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 6 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: 30 minutes (at 37ºC)

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

Kit contents

1 x 1 mL TissueSpec® Intestine dECM

1 x 1 mL 10x Buffer

Workflow overview

Graphical workflow

Material properties

Origin: Intestine (porcine)

Composition: Characterized by mass spectrometry

Stiffness: ##

Usage

Use: 2D cell culture

Preparation temperature: 25ºC

Preparation time: 15 min

Hydrogel volume: 10 mL

Number of wells: 1 x 6 well plate at 1.5 mL per well (calculator)

Storage: 2–8ºC

Shelf life: 12 months

Validated cell types: iPSC-derived human intestine organoids, mouse intestinal crypts, CaCo2 cells, fibroblasts, HUVECs, mesenchymal stem cells

Validated multi-well formats: 6, 12, 24, 48, 96, 384

Validated imaging modalities: Brightfield, phase-contrast, immunofluorescence

Applications: Drug testing, disease modeling, high throughput screening

Quality

Biocompatibility: >95% viability human primary fibroblasts

Sterility: Negative for bacteria, fungus, mycoplasma

Concentration: 0.1 mg/mL (recommended working concentration)

pH: Neutral

Gelation time: N/A

Ordering

Lead time: 5 business days

Shipping temperature: 4ºC (wet ice, natural insulating material)

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