Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC) heterospheroids were embedded within healthy and custom TissueSpec® Human Liver Fibrotic dECM hydrogel, and patient-derived Kupffer cells were seeded on top of the gels. Collagen I was used as the control, non-tissue specific substrate. Cultures were treated with 5 ng/mL TGF-β1 over 3 days. Metabolic activity was measured via PrestoBlue™ assay.

Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC) heterospheroids were embedded within healthy and custom TissueSpec® Human Liver Fibrotic dECM hydrogel, and Kupffer cells were seeded on top of the gels. Collagen I was used as the control, non-tissue specific substrate. Cultures were treated with 5 ng/mL TGF-β1 over 3 days. 

Supernatants were collected and co-cultures were supplemented each day with fresh cell media with or without TGF-β1. CTGF concentrations were quantified by ELISA. Presented values represent the cumulative CTGF secretion per spheroid over the 3-day InMatrico® culture period.

Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC) heterospheroids were embedded within healthy and custom TissueSpec® Human Liver Fibrotic dECM, and Kupffer cells were seeded on top of the gels. Collagen I was used as the control, non-tissue specific substrate. Cultures were treated with 5 ng/mL TGF-β1 over 3 days. 

Supernatants were collected and co-cultures were supplemented each day with fresh cell media with or without TGF-β1. Pro-collagen I concentrations were quantified by ELISA. Presented values represent the cumulative Pro-Collagen I secretion per spheroid over the 3-day InMatrico® culture period.

Representative images of the morphology of heterospheroids (Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC)) embedded within custom TissueSpec® Human Liver Fibrotic dECM hydrogel, healthy liver dECM hydrogel and Collagen I. Kupffer cells (KC) were seeded on top of the gels (not shown). Co-cultures were incubated for 3 days with or without 5 ng/mL TGF-β1. Scale bar = 200 µm.

Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC) heterospheroids were embedded within healthy and custom TissueSpec® Human Liver Fibrotic dECM hydrogel, and patient-derived Kupffer cells were seeded on top of the gels. Collagen I was used as the control, non-tissue specific substrate. Cultures were treated with 5 ng/mL TGF-β1 over 3 days. Metabolic activity was measured via PrestoBlue™ assay.

Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC) heterospheroids were embedded within healthy and custom TissueSpec® Human Liver Fibrotic dECM hydrogel, and Kupffer cells were seeded on top of the gels. Collagen I was used as the control, non-tissue specific substrate. Cultures were treated with 5 ng/mL TGF-β1 over 3 days. 

Supernatants were collected and co-cultures were supplemented each day with fresh cell media with or without TGF-β1. CTGF concentrations were quantified by ELISA. Presented values represent the cumulative CTGF secretion per spheroid over the 3-day InMatrico® culture period.

Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC) heterospheroids were embedded within healthy and custom TissueSpec® Human Liver Fibrotic dECM, and Kupffer cells were seeded on top of the gels. Collagen I was used as the control, non-tissue specific substrate. Cultures were treated with 5 ng/mL TGF-β1 over 3 days. 

Supernatants were collected and co-cultures were supplemented each day with fresh cell media with or without TGF-β1. Pro-collagen I concentrations were quantified by ELISA. Presented values represent the cumulative Pro-Collagen I secretion per spheroid over the 3-day InMatrico® culture period.

Representative images of the morphology of heterospheroids (Hepatocytes-Hepatic Stellate Cells (HepaRG-HSC)) embedded within custom TissueSpec® Human Liver Fibrotic dECM hydrogel, healthy liver dECM hydrogel and Collagen I. Kupffer cells (KC) were seeded on top of the gels (not shown). Co-cultures were incubated for 3 days with or without 5 ng/mL TGF-β1. Scale bar = 200 µm.