TissueSpec® Bone dECM Kits bring the bone matrix environment derived from bones to your cell culture.

Each TissueSpec® dECM Hydrogel Kit is lot-to-lot consistent and is sufficient to prepare 1 mL of hydrogel at a concentration of 6 mg/mL. Research use only.

Kit contents include:

2 x 0.3 mL TissueSpec® dECM

1 x 1 mL Component A

1 x 1 mL  Component B

TissueSpec® Bone dECM Hydrogel (6 mg/mL) was used to model breast-to-bone metastasis in 3D with triple-negative BT549 human breast cancer spheroids embedded in bone-derived extracellular matrix for 72 hours. Scale bar = 100 µm.

Alkaline phosphatase activity of primary human osteoblasts in TissueSpec® Bone dECM Hydrogel, Matrigel, and collagen I. Primary human osteoblasts at passage 3 were embedded in Bone dECM Hydrogel (6 mg/mL), Matrigel (6 mg/mL), or collagen I (6 mg/mL) at a density of 3 x 10^4 cells/mL and 200 µL/well were added to a 24-well plate.

Primary human osteoblasts at passage 3 were embedded in TissueSpec® Bone dECM Hydrogel (6 mg/mL), Matrigel (6 mg/mL), or collagen I (6 mg/mL) at a density of 3 x 10^4 cells/mL and 200 µL/well were added to a 24-well plate. At 7 days, morphology of live cells was imaged using brightfield microscopy at 10x objective. Scale bar: 100 µm.

Cell viability of primary human osteoblasts in TissueSpec® Bone dECM Hydrogel, Matrigel, and collagen I, and no ECM (plastic).

Morphology of primary human osteoblasts in TissueSpec® Bone dECM Hydrogel, Matrigel, and collagen I. Primary human osteoblasts at passage 3 were embedded in Bone dECM Hydrogel (6 mg/mL), Matrigel (6 mg/mL), or collagen I (6 mg/mL) at a density of 3 x 10^4 cells/mL and 200µL/well were added to a 24-well plate. After 6 hours, morphology of live cells was imaged using brightfield microscopy at 10x objective. Scale bar: 100 µm.

Migration activity of breast cancer cells BT-549 to TissueSpec® Bone dECM Hydrogel, Matrigel, and no ECM (plastic). BT-549 cells were seeded on inserts (8.0 µm pore size) placed on top of 24-well plates and previously prepared with 100 µL of thin gels of TissueSpec® Bone dECM Hydrogel (6 mg/mL) or Matrigel (6 mg/mL) and no ECM. After 24 hours, lower chambers were imaged to evaluate migrated cells.

TissueSpec® Bone dECM Kits bring the bone matrix environment derived from bones to your cell culture.

Each TissueSpec® dECM Coating Kit is lot-to-lot consistent and is sufficient to prepare 10 mL of coating at a concentration of 0.1mg/mL. Research use only.

Kit contents include:

1 x 1mL TissueSpec® Bone ECM

1 x 1mL 10x Buffer

Primary human osteoblasts (5 x 10^4) were seeded on 12-well plates previously coated using the TissueSpec® Bone dECM Coating Kit at 0.2 mg/mL for 48 hours. Scale bar: 50 μm.

Lung adenocarcinoma cells (5 x 10^4) were seeded on 12-well plates previously coated using TissueSpec® Bone dECM Coating Kits at 0.2 mg/mL for 24 hours.

BT-549 breast cancer cells (5 x 10^4) were seeded on TissueSpec® Bone dECM Coating prepared at 0.2 mg/mL for 48 hours. Purification of total RNA was carried out using Qiagen RNeasy Mini kit. PCR was run to identify the expression of cancer-related genes.